Journal: Tissue Engineering Part A

Article Title: MicroRNA-1 and MicroRNA-206 Improve Differentiation Potential of Human Satellite Cells: A Novel Approach for Tissue Engineering of Skeletal Muscle

doi: 10.1089/ten.tea.2011.0191

Figure Lengend Snippet: FIG. 1. Satellite cell culture, differentiation, and characterization. During passages 8–15, cultured cells display a homoge- neous morphology, note the triangular-shaped cells that are typical for satellite cells, by differential interference contrast (DIC) microscopy (A). An immunofluorescent image of proliferating cells at passage 9 double stained for a satellite cell marker desmin (green) and a fibroblast marker MCA1399G (red). Nuclei are counterstained with DAPI (blue). More than 95% of the cells expressed the satellite cell marker desmin (B). Differentiation of confluent satellite cell cultures was induced by switching to differentiation medium. Five days after switching to differentiation medium, myotubes were clearly visible by DIC microscopy (C). All scale bars are 100 mm. Color images available online at www.liebertonline.com/tea

Article Snippet: The primary antibody consisted of either (1) a myogenic marker, rabbit-anti-human desmin (1:100; Novus Biological), (2) a fibroblast marker, mouse anti-human MCA1399G (1:100; AbD Serotec), (3) a sarcomere component, mouse-anti-human a-sarcomeric actin IgM (1:200; clone Alpha Sr-1; Abcam) (4) a myogenic transcription factor, mouse-anti-human MyoD (1:100; Dako), (5) a sarcomere component, mouse-anti-human myosin (MF20; 1:500), and (6) a satellite cell marker, mouse-anti-human Pax7 (1:10; both Developmental Studies Hybridoma Bank).

Techniques: Cell Culture, Microscopy, Staining, Marker

Journal: Polymers

Article Title: Innovative Electrospun Nanofiber Mats Based on Polylactic Acid Composited with Silver Nanoparticles for Medical Applications

doi: 10.3390/polym16030409

Figure Lengend Snippet: Percentage of cell viability of L929 mouse fibroblast cells by MTT assay of different mats over 24 h of culture. NC: Cells and culture media without any samples. The experiments were repeated in triplicate.

Article Snippet: L-929 cells, a mouse fibroblast cell line (Elabscience, Houston, TX, USA), were cultured in 96-well plates at a density of 7.5 × 10 4 cells/mL in a completed MEM medium containing 10% FBS and 1% penicillin–streptomycin ( v / v ) and incubated for 24 h in a humidified atmosphere of 5% CO 2 in an incubator at 37 °C.

Techniques: MTT Assay